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KMID : 0545120050150020388
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 2 p.388 ~ p.394
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Distribution of Dominant Bifidobacteria in the Intestinal Microflora of Korean Adults and Seniors, Identified by SDS-PAGE of Whole Cell Proteins and 16S rDNA Sequence Analysis
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Kim TW
Song HS/Kim HY
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Abstract
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In order to investigate the distribution of dominant Bifidobacterium species in intestinal microflora of Korean adults and seniors SDS-PAGE profiles of whole cell proteins were used for the identification of bifidobacteria. To confirm the reliability of SDS-PAGE the Bifidobacterium species identified by SDS-PAGE of whole cell proteins were validated by using 16S rDNA sequencing analysis. The results of SDSPAGE corresponded well with those determined by the analysis of 16S rDNA sequencing. Based on the analysis of SDS-PAGE patterns on unidentified fecal strains which showed positive in fructose-6-phosphate phosphoketolase activity B. adolescentis B. longum and B. bifidum were identified in the feces of adults and B. adolescentis B. longum B. bifidum B. breve and B. dentium were identified in those of seniors. In most of the fecal samples tested the predominant Bifidobacterium species consisted of only a few species and differences in the distribution and numbers of Bifidobacterium species were observed between adults and seniors. B. adolescentis and B. longum were found to be the most common species in feces of adults but not in seniors. Accordingly the distribution and abundance of bifidobacteria in the human intestinal microflora varied depending on the age of hosts.
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